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pbs t vector  (TaKaRa)


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    Structured Review

    TaKaRa pbs t vector
    Pbs T Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+t+vector/PBS+(Phosphate+Buffered+Salts)+Tablets/pmc03199252-198-7-9
    Average 95 stars, based on 186 article reviews
    pbs t vector - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Purification:

    Article Title: MHC polymorphism and disease resistance to vibrio anguillarum in 8 families of half-smooth tongue sole ( Cynoglossus semilaevis )
    Article Snippet: The fragments of interest were excised and purified with the QIAEX II gel extraction kit (Qiagen). .. The purified fragments were cloned into a PBS-T vector (Takara) according to the standard PBS-T vector protocol (Takara) and then transformed into TOP 10 Escherichia coli competent cells (TransGen Biotech). ..

    Clone Assay:

    Article Title: MHC polymorphism and disease resistance to vibrio anguillarum in 8 families of half-smooth tongue sole ( Cynoglossus semilaevis )
    Article Snippet: The fragments of interest were excised and purified with the QIAEX II gel extraction kit (Qiagen). .. The purified fragments were cloned into a PBS-T vector (Takara) according to the standard PBS-T vector protocol (Takara) and then transformed into TOP 10 Escherichia coli competent cells (TransGen Biotech). ..

    Article Title: MHC polymorphism and disease-resistance to Edwardsiella tarda in six turbot (Scophthalmus maximus) families
    Article Snippet: PCR products were electrophoresed on 1.5% agarose gels and the expected amplified fragments were excised before being purified with a QIAEX II gel extraction kit (Qiagen, Hilden, Germany). .. PCR amplicons were cloned into the PBS-T vector (Takara Bio, Otsu, Japan) and propagated in TOP 10 Escherichia coli competent cells (TransGen Biotech) following manufacturer’s instructions. .. Vector specific sequencing primers M13 (5′-CGCCAGGGTTTTCCCAGTCACGAC-3′) and RV-M (5′-AGCGGATAACAATTTCACACAGG-3′), were used to confirm positive clones by PCR.

    Article Title: Aldehyde dehydrogenase 2 protects human umbilical vein endothelial cells against oxidative damage and increases endothelial nitric oxide production to reverse nitroglycerin tolerance.
    Article Snippet: .. Eukaryotic expression vector construction Total RNA was extracted from normal human hepatocytes and used to synthesize ALDH2 complementary DNA (cDNA) by reverse transcription polymerase chain reaction (PCR). cDNA was then cloned into the pBS-T vector with PrimeSTAR HS DNA polymerase (TaKaRa, Dalian, China). ..

    Article Title: SCAR molecular markers of the B biotype and two non-B populations of the whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)
    Article Snippet: Random amplified polymorphic DNA (RAPD) analyses were performed with random primer H16 for the B biotype and two non-B populations of Bemisia tabaci collected from Zhejiang (China).. The specific sequence fragments containing 446, 390 and 1317 nucleotides were amplified for the B biotype, ZHJ-1, ZHJ-2 populations, respectively.. The three specific fragments were cloned and sequenced, and three pairs of SCAR primers were designed according to the sequences determined.

    Article Title: The Function and Mechanism of Pathogenesis-Related 5 Protein Resistance in Cherry Tomato in Response to Alternaria alternata
    Article Snippet: Pathogenesis-related protein 5 (PR5) is encoded by the host and is related to host resistance to biological and abiotic factors.. In the present study, we constructed interference expression vector pBIA-LePR5-RNAi and analyzed the resistance of transgenic tomatoes to Alternaria alternata (A. alternata).. To understand the regulatory mechanism of LePR5 gene, green fluorescent protein fluorescence cellular localization and promoter functional elements were investigated.

    Plasmid Preparation:

    Article Title: MHC polymorphism and disease resistance to vibrio anguillarum in 8 families of half-smooth tongue sole ( Cynoglossus semilaevis )
    Article Snippet: The fragments of interest were excised and purified with the QIAEX II gel extraction kit (Qiagen). .. The purified fragments were cloned into a PBS-T vector (Takara) according to the standard PBS-T vector protocol (Takara) and then transformed into TOP 10 Escherichia coli competent cells (TransGen Biotech). ..

    Article Title: MHC polymorphism and disease-resistance to Edwardsiella tarda in six turbot (Scophthalmus maximus) families
    Article Snippet: PCR products were electrophoresed on 1.5% agarose gels and the expected amplified fragments were excised before being purified with a QIAEX II gel extraction kit (Qiagen, Hilden, Germany). .. PCR amplicons were cloned into the PBS-T vector (Takara Bio, Otsu, Japan) and propagated in TOP 10 Escherichia coli competent cells (TransGen Biotech) following manufacturer’s instructions. .. Vector specific sequencing primers M13 (5′-CGCCAGGGTTTTCCCAGTCACGAC-3′) and RV-M (5′-AGCGGATAACAATTTCACACAGG-3′), were used to confirm positive clones by PCR.

    Article Title: Cloning and characterization of two Argonaute genes in wheat ( Triticum aestivum L.)
    Article Snippet: .. After the products were separated by 1% agarose gel electrophoresis, their bands were recovered and sub-cloned into the pBS-T vector (TakaRa, China) for sequencing. .. The RACE technique was performed with a TaKaRa kit (5 ′ -Full RACE Kit and 3 ′ -Full RACE Core Set Ver.

    Article Title: Aldehyde dehydrogenase 2 protects human umbilical vein endothelial cells against oxidative damage and increases endothelial nitric oxide production to reverse nitroglycerin tolerance.
    Article Snippet: .. Eukaryotic expression vector construction Total RNA was extracted from normal human hepatocytes and used to synthesize ALDH2 complementary DNA (cDNA) by reverse transcription polymerase chain reaction (PCR). cDNA was then cloned into the pBS-T vector with PrimeSTAR HS DNA polymerase (TaKaRa, Dalian, China). ..

    Article Title: The Function and Mechanism of Pathogenesis-Related 5 Protein Resistance in Cherry Tomato in Response to Alternaria alternata
    Article Snippet: Pathogenesis-related protein 5 (PR5) is encoded by the host and is related to host resistance to biological and abiotic factors.. In the present study, we constructed interference expression vector pBIA-LePR5-RNAi and analyzed the resistance of transgenic tomatoes to Alternaria alternata (A. alternata).. To understand the regulatory mechanism of LePR5 gene, green fluorescent protein fluorescence cellular localization and promoter functional elements were investigated.

    Transformation Assay:

    Article Title: MHC polymorphism and disease resistance to vibrio anguillarum in 8 families of half-smooth tongue sole ( Cynoglossus semilaevis )
    Article Snippet: The fragments of interest were excised and purified with the QIAEX II gel extraction kit (Qiagen). .. The purified fragments were cloned into a PBS-T vector (Takara) according to the standard PBS-T vector protocol (Takara) and then transformed into TOP 10 Escherichia coli competent cells (TransGen Biotech). ..

    Article Title: The Function and Mechanism of Pathogenesis-Related 5 Protein Resistance in Cherry Tomato in Response to Alternaria alternata
    Article Snippet: Pathogenesis-related protein 5 (PR5) is encoded by the host and is related to host resistance to biological and abiotic factors.. In the present study, we constructed interference expression vector pBIA-LePR5-RNAi and analyzed the resistance of transgenic tomatoes to Alternaria alternata (A. alternata).. To understand the regulatory mechanism of LePR5 gene, green fluorescent protein fluorescence cellular localization and promoter functional elements were investigated.

    Polymerase Chain Reaction:

    Article Title: MHC polymorphism and disease-resistance to Edwardsiella tarda in six turbot (Scophthalmus maximus) families
    Article Snippet: PCR products were electrophoresed on 1.5% agarose gels and the expected amplified fragments were excised before being purified with a QIAEX II gel extraction kit (Qiagen, Hilden, Germany). .. PCR amplicons were cloned into the PBS-T vector (Takara Bio, Otsu, Japan) and propagated in TOP 10 Escherichia coli competent cells (TransGen Biotech) following manufacturer’s instructions. .. Vector specific sequencing primers M13 (5′-CGCCAGGGTTTTCCCAGTCACGAC-3′) and RV-M (5′-AGCGGATAACAATTTCACACAGG-3′), were used to confirm positive clones by PCR.

    Article Title: Aldehyde dehydrogenase 2 protects human umbilical vein endothelial cells against oxidative damage and increases endothelial nitric oxide production to reverse nitroglycerin tolerance.
    Article Snippet: .. Eukaryotic expression vector construction Total RNA was extracted from normal human hepatocytes and used to synthesize ALDH2 complementary DNA (cDNA) by reverse transcription polymerase chain reaction (PCR). cDNA was then cloned into the pBS-T vector with PrimeSTAR HS DNA polymerase (TaKaRa, Dalian, China). ..

    Agarose Gel Electrophoresis:

    Article Title: Cloning and characterization of two Argonaute genes in wheat ( Triticum aestivum L.)
    Article Snippet: .. After the products were separated by 1% agarose gel electrophoresis, their bands were recovered and sub-cloned into the pBS-T vector (TakaRa, China) for sequencing. .. The RACE technique was performed with a TaKaRa kit (5 ′ -Full RACE Kit and 3 ′ -Full RACE Core Set Ver.

    Article Title: SCAR molecular markers of the B biotype and two non-B populations of the whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)
    Article Snippet: Random amplified polymorphic DNA (RAPD) analyses were performed with random primer H16 for the B biotype and two non-B populations of Bemisia tabaci collected from Zhejiang (China).. The specific sequence fragments containing 446, 390 and 1317 nucleotides were amplified for the B biotype, ZHJ-1, ZHJ-2 populations, respectively.. The three specific fragments were cloned and sequenced, and three pairs of SCAR primers were designed according to the sequences determined.

    Sequencing:

    Article Title: Cloning and characterization of two Argonaute genes in wheat ( Triticum aestivum L.)
    Article Snippet: .. After the products were separated by 1% agarose gel electrophoresis, their bands were recovered and sub-cloned into the pBS-T vector (TakaRa, China) for sequencing. .. The RACE technique was performed with a TaKaRa kit (5 ′ -Full RACE Kit and 3 ′ -Full RACE Core Set Ver.

    Expressing:

    Article Title: Aldehyde dehydrogenase 2 protects human umbilical vein endothelial cells against oxidative damage and increases endothelial nitric oxide production to reverse nitroglycerin tolerance.
    Article Snippet: .. Eukaryotic expression vector construction Total RNA was extracted from normal human hepatocytes and used to synthesize ALDH2 complementary DNA (cDNA) by reverse transcription polymerase chain reaction (PCR). cDNA was then cloned into the pBS-T vector with PrimeSTAR HS DNA polymerase (TaKaRa, Dalian, China). ..

    Reverse Transcription:

    Article Title: Aldehyde dehydrogenase 2 protects human umbilical vein endothelial cells against oxidative damage and increases endothelial nitric oxide production to reverse nitroglycerin tolerance.
    Article Snippet: .. Eukaryotic expression vector construction Total RNA was extracted from normal human hepatocytes and used to synthesize ALDH2 complementary DNA (cDNA) by reverse transcription polymerase chain reaction (PCR). cDNA was then cloned into the pBS-T vector with PrimeSTAR HS DNA polymerase (TaKaRa, Dalian, China). ..

    Gel Extraction:

    Article Title: SCAR molecular markers of the B biotype and two non-B populations of the whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)
    Article Snippet: Random amplified polymorphic DNA (RAPD) analyses were performed with random primer H16 for the B biotype and two non-B populations of Bemisia tabaci collected from Zhejiang (China).. The specific sequence fragments containing 446, 390 and 1317 nucleotides were amplified for the B biotype, ZHJ-1, ZHJ-2 populations, respectively.. The three specific fragments were cloned and sequenced, and three pairs of SCAR primers were designed according to the sequences determined.



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